5-BROMO-6-CHLORO-3-INDOXYL OCTANOATE - Names and Identifiers
5-BROMO-6-CHLORO-3-INDOXYL OCTANOATE - Physico-chemical Properties
Molecular Formula | C16H19BrClNO2
|
Molar Mass | 372.68 |
Density | 1.397±0.06 g/cm3(Predicted) |
Melting Point | 57-62°C |
Boling Point | 482.6±40.0 °C(Predicted) |
Flash Point | 245.687°C |
Vapor Presure | 0mmHg at 25°C |
pKa | 13.70±0.30(Predicted) |
Storage Condition | Sealed in dry,2-8°C |
Refractive Index | 1.592 |
MDL | MFCD00798386 |
Use | Suitable for the detection of esterase. |
5-BROMO-6-CHLORO-3-INDOXYL OCTANOATE - Risk and Safety
WGK Germany | 3 |
FLUKA BRAND F CODES | 10 |
5-BROMO-6-CHLORO-3-INDOXYL OCTANOATE - Introduction
octanoate, also known as BCIP (hexanoate), is an organic compound. The following is an introduction to the nature, use, formulation and safety information of BCIP:
Nature:
-Appearance: BCIP is white or yellowish crystalline powder.
-Solubility: BCIP is easy to dissolve in organic solvents (such as dimethyl sulfoxide and ethyl acetate), but it is less soluble in water.
-Stability: BCIP is a relatively stable compound, but it will gradually decompose under bright light.
Use:
-biochemical experiments: BCIP is a substrate for biochemical experiments, which is often used to detect the activity of alkaline phosphatase in enzyme labeling technology. When alkaline phosphatase is present, BCIP is hydrolyzed by it to produce a visible purple product.
-In vitro tissue staining: BCIP is often used as a stain for in vitro tissue staining to detect the activity of alkaline phosphatase and the distribution of specific proteins in cells or tissues.
Method:
The preparation of BCIP is usually carried out by synthetic chemical methods. The main steps include the synthesis of indole precursors and the reaction with bromine and chlorine compounds to generate the ester structure of BCIP.
Safety Information:
-Protective measures: Wear appropriate protective gloves and glasses when using BCIP to avoid contact with skin and eyes. Avoid inhaling dust or vapors of the compound during operation.
-Storage Note: BCIP should be stored in a dry, cool place, away from fire and oxidizing agents.
-Toxicity: Since BCIP is mainly used in laboratory operations, it is less toxic, but it is still necessary to follow reasonable laboratory safety procedures. Inadvertently ingested or exposed to BCIP should seek medical attention immediately.
Last Update:2024-04-09 21:04:16